Wasn't that easy to dispose the Image, since this
Code:
this.pb_map.Image = img;
img.dispose() does not only dispose the img alone but since its referenced also by the pictureBox.Image which still is needed (thats why the invalid paramter exceptions are thrown after dispose() i mentioned before).
Took me a while until i saw that.

Now I'm doing it this way:
Code:
//if no Image exists on Picture Box, create one (looks like the source)
			if (this.pb_map.Image == null)
			{
				this.pb_map.Image = (Image)img.Clone();  //Make it not the Image itself but a clone of it (so im can be disposed)
			}
			g = Graphics.FromImage(this.pb_map.Image); //create Graphics Object
			//draw area
			g.DrawImage(img, x * 32, y * 32, new Rectangle(this.runtime.Selector.X * 32, this.runtime.Selector.Y * 32, 32, 32), GraphicsUnit.Pixel);
			img.Dispose();
Now I can't reproduce the OoM-Exception but to be honest, I can't believe that this solution is the best (I don't like to copy or to clone...) so I'm asking what do you think about this idea?